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HBsAgGi: HB surface antigen glycan isomer

A monoclonal antibody to O-glycosylated PreS2 on M-HBs, which is predominantly expressed by DNA-containing viral particle.

Overview: HBV envelope protein is composed of three types of surface antigens, S-, M-, and L-HBs, which are produced from an HBsAg gene containing PreS1, PreS2 and S-domains1. HBsAgs are heavily glycosylated with N-glycan and O-glycan2,3. Whole glycan structural analyses revealed that PreS2 domain on M-HBs, but not on L-HBs, contains highly conserved O-glycosylated site in genotype C (gC)4,5. A recombinant monoclonal antibody against HBsAg glycan isomer (HBsAgGi) was generated by using O-glycosylated PreS2 peptide. Compared to traditional HBsAg-testing which recognizes all viral particles, HBsAgGi specifically recognizes infectious HBV particles (DNA virion).

 Isotype: mouse IgG1
 Light chain: kappa
 Amount: 50 μg
 Storage:Store at 4°C for short term. Upon delivery store at  -20°C for long term and avoid freeze / thaw cycle.

Applications:
 1. Western
 2. Immunoprecipitation/Separation of HBV particles
 3. ELISA
 4. HBV infection inhibition assay
 5. Immunohistochemistry

Examples:

Western blotting using HBsAgGi (A) and PreS2 antibody (B)

  • A: HBsAgGi-gC antibody, B: anti-PreS2 antibody
  • Lane 1: S-HBsAg 50 ng
  • Lane 2: M-HBsAg 50 ng
  • Lane 3: L-HBsAg 50 ng
  • Secondary antibody: HRP -labeled anti-mouse IgG

ELISA using HBsAgGi-gC coated plate and M-HBsAg as a standard sample

  • Standard material: M-HBsAg (3.7 – 900 ng/mL)
  • Tested samples: sera from patients with HBV (2 μL)
  • Serum #1: HBsAg 6610 IU/mL, HBV DNA 3.1
  • Serum #2: HBsAg 8612 IU/mL, HBV DNA 0
  • Serum #3: HBsAg 36600 IU/mL, HBV DNA 8.3
  • Detection: Biotinylated HBsAgGi-gC antibody and HRP-labeled streptavidin

Immunoprecipitation using HBsAgGi-gC

  • Original sample (Orig): HBV patient serum (4.1 logIU/20 μL)
  • IP condition: Biotinylated HBsAgGi-gC 2 μg, Streptavidin-conjugated magnetic beads 10 μL/1 mL TBS-T, 4°C, 16 hours
  • Quantification of HBV DNA: HBV DNA per 20 μL from precipitated fraction (IP) and supernatant fraction (Sup) was measured by real-time PCR method.

References:

1. Schadler and Hildt (2009) Viruses 1:185-209.
2. Schmitt et al. (2004) J Gen Virol 85:2045-2053.
3. Dobrica et al. (2020) Cells 9:1404.
4. Wagatsuma et al. (2018) Anal Chem 90:10196-10203.
5. Angata et al. (2021) Biochim Biophys Acta Gen Subj. In Press.


Download a leaflet for HBsAgGi

HBsAgGi: recombinant mouse IgG1, 50 μg, US$700

To purchase HBsAgGi, please contact marketing@rcmg-glyco.com

RCMG Inc.


2-1-6 Sengen, Plaza Suite 106, Tsukuba, Ibaraki 305-0047
TEL:+81-29-828-8010
EMAIL : marketing@rcmg-glyco.com

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